Stereoscopic Medical Data Video Quality Issues

Stereoscopic medical videos are recorded, e.g., in stereo endoscopy or during video recording medical/dental operations. This paper examines quality issues in the recorded stereoscopic medical videos, as insufficient quality may induce visual fatigue to doctors. No attention has been paid to stereo quality and ensuing fatigue issues in the scientific literature so far. Two of the most commonly encountered quality issues in stereoscopic data, namely stereoscopic window violations and bent windows, were searched for in stereo endoscopic medical videos. Furthermore, an additional stereo quality issue encountered in dental operation videos, namely excessive disparity, was detected and fixed. The conducted experiments prove the existence of such quality issues in stereoscopic medical data and highlight the need for their detection and correction

Statistical method of context evaluation for biological sequence similarity

Within this paper we are proposing and testing a new strategy for detection and measurement of similarity between sequences of proteins. Our approach has its roots in computational linguistics and the related techniques for quantifying and comparing content in strings of characters. The pairwise comparison of proteins relies on the content regularities expected to uniquely characterize each sequence. These regularities are captured by n-gram based modelling techniques and exploited by cross-entropy related measures. In this new attempt to incorporate theoretical ideas from computational linguistics into the field of bioinformatics, we experimented using two implementations having always as ultimate goal the development of practical, computationally efficient algorithms for expressing protein similarity. The experimental analysis reported herein provides evidence for the usefulness of the proposed approach and motivates the further development of linguistics-related tools as a means of analysing biological sequences.IFIP International Conference on Artificial Intelligence in Theory and Practice - Integration of AI with other TechnologiesRed de Universidades con Carreras en Informática (RedUNCI

Separated Instrument in Endodontics: Frequency, Treatment and Prognosis

Instrument separation during endodontic therapy is a frequent accident with rotary instruments being more likely to separate than manual ones. The treatment of cases with a separated instrument can be either conservative or surgical. A conservative approach involves the following treatment choices: a) bypass of the fragment, b) removal of the fragment, c) instrumentation and obturation coronally to the fragment. Concerning the removal of a separated instrument, a variety of techniques and systems have been developed. Ultrasonics, in combination with the operative microscope constitute the most effective and reliable tools for removing a separated endodontic instrument from a root canal. The likelihood of successful removal depends on: the level of separation (coronal, middle or apical third); location in relation to the root canal curvature; the type of separated instrument; its length; the degree of canal curvature and the tooth type. Several complications may occur during the management of a separated instrument: separation of the ultrasonic tip or file used for bypassing or removing the instrument; further separation of the fragment; perforation; ledge; extrusion of the file into periapical tissues; tooth weakening due to dentin removal, as well as excessive temperature rise in periodontal tissues. Prognosis for a tooth retaining a separated instrument depends on the presence of a periapical lesion, the microbial load of the root canal during the time of separation and the quality of the obturation

Root canal therapy for the prevention of osteonecrosis of the jaws : an evidence-based clinical update

Osteonecrosis of the jaws is an adverse effect of bone preservation treatment. There is a sufficient body of evidence to associate osteonecrosis of the jaws development with dental extractions and trauma caused from ill-fitting dentures. In this review, we critically appraise available evidence about the clinical efficacy of root canal therapy in patients receiving bisphosphonates. We review a series of theories to explain why endodontic treatment is a safe clinical intervention to prevent osteonecrosis of the jaws in patients receiving bisphosphonates. Root canal therapy could postpone or even eradicate the need for dental extractions of carious teeth in patients on bisphosphonates who may develop osteonecrosis of the jaws. Patients receiving bisphosphonates should be offered the full range of preventive care to reduce their risk to both dental caries and periodontal disease, so that the need for both endodontic therapy and dental extractions will be reduced. Implementing such a strategy would require both practitioner and patient education through the combined efforts of medical and dental societies. Such an approach is justified, as the risk of compromising the oral health of patients on bisphosphonates undertaking endodontic treatment is negligible compared with the benefit from avoiding dental extractions

3D reconstruction of two C-shape mandibular molars

Two mandibular second molars, with an indication of C-shape morphology were processed for 3-D reconstruction. After serial cross sectioning, photographs of the sections were digitized and by using surface representation, 3D reconstruction was achieved. The first molar as the 3D reconstruction showed was single rooted with one C-shaped root canal with two foramens, while the second one was double rooted with two root canals, one C-shaped and one thin, having a common foramen

Computerized Reconstruction of Pulpal Blood Vessels Examined under Confocal Microscope

The purpose of this study was the evaluation of 3 different histological methods for studying pulpal blood vessels in combination with 2 types of confocal microscope and computer assisted 3-dimensional reconstruction. 10 human, healthy, free of restorations or caries teeth that were extracted for orthodontic reasons were used. From these teeth, the pulp tissues of 5 were removed, fixed in formalin solution, dehydrated and embedded in paraffin. Serial cross sections 5μm thick were taken from 3 of the above mentioned pulpal tissues and stained with CD34 according to the immunohistochemical ABC technique, while the rest 2 were stained with CD34 and Cy5 by means of immunofluorescence after serial cross sectioning of 10μm. 5 of the 10 teeth were fixed, decalcified, serial cross sectioned (30μm thickness) and stained with eosin. The physical sections were examined under 2 types of confocal laser microscope. Serial images were taken for each section, alignment of the images was followed and finally 3-dimensional reconstructions of the pulpal vessels were achieved

THE BREAST Evaluation of FISH image analysis system on assessing HER2 amplification in breast carcinoma cases

Abstract HER2-positive breast cancer is characterized by aggressive growth and poor prognosis. Women with metastatic breast cancer with over-expression of HER2 protein or excessive presence of HER2 gene copies are potential candidates for Herceptin (Trastuzumab) targeted treatment that binds to HER2 receptors on tumor cells and inhibits tumor cell growth. Fluorescent in situ hybridization (FISH) Q1 is one of the most widely used methods to determine HER2 status. Typically, evaluation of FISH images involves manual counting of FISH signals in multiple images, a time consuming and error prone procedure. Recently, we developed novel software for the automated evaluation of FISH images and, in this study, we present the first testing of this software on images from two separate research clinics. To our knowledge, this is the first concurrent evaluation of any FISH image analysis software in two different clinics. The evaluation shows that the developed FISH image analysis software can accelerate evaluation of HER2 status in most breast cancer cases.